Assassin

An assassin is a person who is trained to kill other people, normally for political or personal reasons. Oftentimes they work as independent agents for hire.

In One Piece
The Baroque Works agents were known to have assassins in Whiskey Peak. Mr. 5 and Miss Valentine tried to murder Princess Vivi but failed. Mr. 3 tried to kill the Straw Hat Pirates, Vivi, Dorry, and Brogy but also failed.

Nico Robin claimed that she is an assassin when Usopp interviewed her upon her entry into the Straw Hat crew, but her targets and reasons have yet to be revealed. However, she was prepared to kill Crocodile since he recruited her into Baroque Works, and tried it when he declared that he was going to kill her, though her attempt ultimately failed.

The CP9 are the first assassins in the series to work for the World Government and they are the only known World Government assassins. Lucci, Kalifa, Blueno, and Kaku were assigned to Water 7 to get the Pluton blueprints and to kill Iceburg. Meanwhile, Jabra, Fukuro, and Kumadori were sent to an unnamed town to assassinate three key Revolutionary leaders (Dragon not included). However, they ended up killing twenty extra people thanks to Fukuro revealing his team's presence and their intentions to the town. This strongest generation has been dismissed as criminals when their superior, Spandam, framed them of a debacle at the Enies Lobby.

Hyouzou is a mercenary hired by the New Fishman Pirates to become an assassin of the crew. His profession came to an end when he overdosed on Energy Steroids, which eventually stripped him of all of his natural prowess, rendering him a frail and weak old man, and the fact that he was arrested and put in the prison of the Ryugu Palace.

The Yeti Cool Brothers are Caesar Clown's enforcers tasked to search for the Straw Hat Pirates, hunt them down and extinguish them with extreme prejudice.

Baby 5 is an assassin who doubles as a maid for the Donquixote Pirates. Violet is also an assassin for the same crew, using her act as a street dancer as a coverup.

The Funk Brothers are assassins from Mogaro Kingdom.

The Vinsmoke Family is a clan of assassins, infamous throughout the New World. They consist of a father and at least three sons, the third of whom is Sanji.

Trivia

 * Kuro can reportedly kill 50 highly skilled assassins before they notice his presence.
 * Hyouzou was referred as a "Manslayer" (人斬) by the New Fishman Pirates members, a samurai who specializes in assassinations.

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Assassin