Splash and Splatter

Splash and Splatter are two okama pirate twins who are found on Fishman Island. They both willingly donated blood to Sanji, who was dying of severe blood loss.

Appearance
They are both muscular and have exaggerated male facial features, of which are covered with excessive make-up. They have spiked eyebrows and long spiked hair. They wear the Criminal brand and are almost always constantly flexing. One wears pink and has brown hair, while the other wears white and has blonde hair. In Gigant Battle 2, they wear purple and yellow.

Personality
Splash and Splatter both act very effeminate, as any okama would. They also seem to brag constantly about their physique by showing off their muscles. They seem to be nice individuals as they willingly gave large amounts of their blood to save a fellow human life. They end all of their sentences with the word "silly", followed by a heart, showing the "feminine tone" being used in speech.

History
They have apparently been on Fishman Island for a long time way before the Straw Hats arrive since Keimi stated that Luffy and his crew are the first visitors in a while. Another hint on their long stay on island is that Splash and Splatter are not under the clutches of the New Fishman Pirates while Gyro and his crew were not so lucky.

When Sanji was suffering critical blood loss, Splash and Splatter volunteered to donate blood to him. When Sanji regained consciousness, the two okamas revealed themselves to him, much to Sanji's horror.

Anime and Manga Differences
In the anime they are shown again during a scene when Sanji starts fantasizing about Nami and Robin and is about to lose more blood. Chopper calls Splash and Splatter who appear in pink nurse mini dresses and attempt to treat him. Sanji freaks out upon seeing them which naturally makes him snap out of his perverted dreams and hence prevents another nose bleed.

Trivia

 * They both have S RH- type blood.
 * They make a cameo in Mermaid Cove stage of the game Gigant Battle! 2.

External References

 * Transvestism - Wikipedia article about Transvestism in general
 * Twins - Wikipedia article about twins