|Brief||?????? †||??????||Monkey D. Luffy (Unofficial)|
- †: the character is deceased.
- *: the character's status is unknown, or show additional information. Hovering the symbol may give further details.
- ≠: the character is non-canon.
- ‡: the character is no longer part of this group. Hovering the symbol may give further details.
The only named member of the crew is the captain, Brief. Including him, each of the crew members had the same kind of mustache, but none of the others were ever seen except in a photo. Brief also has a pet goat. It is unknown whether the goat joined before or after the death of the rest of the crew.
Baron Omatsuri and the Secret Island
When Monkey D. Luffy and his crew arrived on the island, Brief watched them from a distance and later got Luffy alone where he offered him to join the Mustache Pirates and defeat Omatsuri. Luffy declined, but Brief warned him about Omatsuri.
Later, he saved Luffy from being killed by Omatsuri. Brief explained the history of his crew to Luffy before the young captain ran off to be defeated yet again by Omatsuri. After strategizing, the two partnered up to distract and defeat Omatsuri, with the help of Papa and the Tearoom Pirates, the former of which who actually killed Lily Carnation. At the end of the movie, Brief acknowledged that he would remember Luffy and would look for a new crew, inspired by the young captain.
- The Mustache Pirates had a group sign to acknowledge that they were all friends, which was putting your finger under your nose to make a finger mustache.
- The crew's identical appearance with their captain is something that rarely happens in the series, only with this crew and Brief and the Caribou Pirates and their captain Coribou.