When it was first seen, SAD was being kept in a large metallic vat hooked up to several tubes and a prominent nuclear symbol on it. After the vat holding the refined SAD was split open by Law, the substance is revealed to be somewhat of a foamy mixture similar to wax.
SAD is a particularly unique substance and it is one of the few things needed to create SMILEs, artificial Zoan Devil Fruits. While successful ones retain the "hammer" weakness other Devil Fruits have, the duds also take away all negative emotions. It has the potential to cause anarchy within the New World, depending on the one who wields it. It also might have some radioactive properties, as the symbol for radioactive substances appeared on the tank Law was looking at; and Law himself noted that the laboratory will soon explode after the state he left the SAD production room in. Caesar Clown is the only person in the world who knows how to manufacture this substance, and he created it via the practical application of Vegapunk's and Vinsmoke Judge's discovery of the universal genetic blueprint: the Lineage Factor. As such, SAD is very valuable, as Donquixote Doflamingo suggested destroying the production room and eliminating Caesar to prevent it from being stolen and the scientist from being kidnapped, only to retract the suggestion as it would be troublesome for him to lose his supply of the substance as well as any chance of procuring more.
Punk Hazard ArcEdit
Trafalgar Law apparently went through great lengths to gain access to the SAD production room on Punk Hazard, joining the Shichibukai and then forming an alliance with the Straw Hat Pirates. He eventually succeeded amidst the chaos in the research facility, but Vergo contacted Doflamingo and reported it. Vergo was ordered to stop Law from taking the SAD and execute him for his betrayal, which leads to the two of them battling in the production room.
After Law and Smoker engaged and defeated Vergo, Law broke the production machines, crippling the production of SAD. Due to this, afterwards the entire room exploded with Vergo inside of it.
Despite the destruction of the production facility in Punk Hazard and Caesar's captivity, the Donquixote Pirates still possessed a decent quantity of SAD in their factory, as the dwarves continued their labor of cultivating SMILEs. Ultimately, the remaining SAD supply, along with the SMILE Factory, were all destroyed when the dwarves rose up in revolt.
- SAD is similar to the Joker Venom from DC Comics, as both were created by someone with the moniker of "Joker" (Joker and Doflamingo) and cause a permanent grin with its victims.
- However, SAD is just an ingredient in the making of SMILE, which causes the uncontrolled grinning in only 90% of the times, while the venom can be used directly and unless the victim is vaccinated, it always works.
- ↑ 1.0 1.1 1.2 One Piece Manga and Anime — Vol. 69 Chapter 690 (p. 17) and Episode 616, The physical form of SAD is seen when Law slices through the production room.
- ↑ 2.0 2.1 2.2 One Piece Manga and Anime — Vol. 68 Chapter 672 (p. 4) and Episode 598, Vergo says he came from Dressrosa on an SAD Tanker.
- ↑ 3.0 3.1 One Piece Manga and Anime — Vol. 69 Chapter 681 (p. 19) and Episode 607, Vergo realizes Law's objective to get to SAD.
- ↑ One Piece Manga and Anime — Vol. 69 Chapter 689 (p. 16) and Episode 615.
- ↑ One Piece Manga — Vol. 94 Chapter 943 (p. 10).
- ↑ 6.0 6.1 One Piece Manga and Anime — Vol. 70 Chapter 698 (p. 16-17) and Episode 625, Law explains what he knows about SAD and SMILEs to the Straw Hats.
- ↑ One Piece Manga and Anime — Vol. 69 Chapter 682 (p. 7-9) and Episode 608.
- ↑ One Piece Manga and Anime — Vol. 74 Chapter 738 (p. 4-7) and Episode 671, Dwarves continued to produce SMILEs.
- ↑ One Piece Manga and Anime — Vol. 78 Chapter 779 (p. 5-6) and Episode 720, The Tontatta dwarves destroy the SMILE Factory.