When it was first seen, SAD was being kept in a large metallic vat hooked up to several tubes and a prominent nuclear symbol on it. After the vat holding the refined SAD was split open by Law, the substance is revealed to be somewhat of a foamy mixture similar to wax.
SAD is a particularly unique substance and it is one of the few things needed to create SMILEs, artificial Zoan Devil Fruits. While successful ones retain the "hammer" weakness other Devil Fruits have, the duds also take away all negative emotions. It has the potential to cause anarchy within the New World, depending on the one who wields it. It also might have some radioactive properties, as the symbol for radioactive substances appeared on the tank Law was looking at; and Law himself noted that the laboratory will soon explode after the state he left the SAD production room in. Caesar Clown is the only person in the world who knows how to manufacture this substance, and he created it via the practical application of Vegapunk's and Vinsmoke Judge's discovery of the universal genetic blueprint: the Lineage Factor. As such, SAD is very valuable, as Donquixote Doflamingo suggested destroying the production room and eliminating Caesar to prevent it from being stolen and the scientist from being kidnapped, only to retract the suggestion as it would be troublesome for him to lose his supply of the substance as well as any chance of procuring more. According to Shinobu, the SAD formula is still incomplete. Thus, the SMILE fruits it produced have an extremely unstable risk of having only a ten percent chance of success in granting artificial Zoan enhancements to consumers with a majority suffering the side effects of losing the ability to express negative emotions.
Punk Hazard ArcEdit
Trafalgar Law apparently went through great lengths to gain access to the SAD production room on Punk Hazard, joining the Shichibukai and then forming an alliance with the Straw Hat Pirates. He eventually succeeded amidst the chaos in the research facility, but Vergo contacted Doflamingo and reported it. Vergo was ordered to stop Law from taking the SAD and execute him for his betrayal, which leads to the two of them battling in the production room.
Despite the destruction of the production facility in Punk Hazard and Caesar's captivity, the Donquixote Pirates still possessed a decent quantity of SAD in their factory, as the dwarves continued their labor of cultivating SMILEs. Ultimately, the remaining SAD supply, along with the SMILE Factory, were all destroyed when the dwarves rose up in revolt.
- SAD is similar to the Joker Venom from DC Comics, as both were created by someone with the moniker of "Joker" (Joker and Doflamingo) and cause a permanent grin with its victims.
- However, SAD is just an ingredient in the making of SMILE, which causes the uncontrolled grinning in only 90% of the times, while the venom can be used directly and unless the victim is vaccinated, it always works.